GE - RPN1606

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Contents

GE Healthcare Amersham Megaprime DNA Labelling Systems Product Booklet Codes RPN1604 RPN1605 RPN1606 RPN1607 Page finder 1 Legal 3 2 Handling 4 2 1 Safety warnings and precautions 4 2 2 Storage and stability 4 2 3 Quality control 4 3 System components 6 3 1 Megaprime DNA labelling systems 8 4 Introduction 9 5 Megaprime DNA labelling protocols 11 5 1 Standard megaprime protocol 11 5 2 New megaprime protocol 15 5 3 Use of alternative reaction conditions 20 6 Appendices 27 6 1 Appendix I Labelling of DNA fragments in low melting point agarose 27 6 2 Appendix II Monitoring the reaction and calculating the specific activity of the labelled DNA 28 6 3 Appendix III Removal of unincorporated nucleotides 32 6 4 Appe ...